![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3059771:Design
Designed by: Beteel Abu-Ageel Group: iGEM19_William_and_Mary (2019-10-21)
Turing Patterns Inhibitor Circuit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1488
Illegal PstI site found at 1926
Illegal PstI site found at 3827 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1488
Illegal PstI site found at 1926
Illegal PstI site found at 3827 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1488
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1488
Illegal PstI site found at 1926
Illegal PstI site found at 3827 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1488
Illegal PstI site found at 1926
Illegal PstI site found at 3827 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2005
Illegal BsaI site found at 3875
Illegal BsaI.rc site found at 2078
Illegal BsaI.rc site found at 4020
Design Notes
I needed to design new sticky ends to allow for all of these parts to come together.
Source
Sources: 1. Karig, D., Martini, K. M., Lu, T., Delateur, N. A., Goldenfeld, N., & Weiss, R. (2018). Stochastic Turing patterns in a synthetic bacterial population. Proceedings of the National Academy of Sciences, 115(26), 6572-6577. doi:10.1073/pnas.1720770115
2. Karig, D. K. (2007). Engineering multi-signal synthetic biological systems. (Doctoral dissertation). Princeton University, USA.